Recombinant
RabMAb

Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400)

Overview

  • Product name
    Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)]
    See all Hormone sensitive lipase primary antibodies
  • Description
    Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase (phospho S853)
  • Specificity
    The antibody only detects HSL phosphorylated on serine 853.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho-specific peptide corresponding to residues surrounding serine 853 of Human HSL/LIPE (UniProt Q05469).

  • Positive control
    • Human skeletal muscle lysates and Mouse muscle lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab109400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

      Lane 1 : Human skeletal muscle lysates
      Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 117 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Hormone sensitive lipase (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

      Lane 1 : Mouse muscle lysate
      Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 117 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

    References

    ab109400 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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