Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228)

Overview

  • Product nameAnti-Hormone sensitive lipase (phospho S855) antibody
    See all Hormone sensitive lipase primary antibodies
  • Description
    Rabbit polyclonal to Hormone sensitive lipase (phospho S855)
  • Specificityab55228 detects endogenous levels of HSL/LIPE only when phosphorylated at serine 554.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human HSL/LIPE around the phosphorylation site of serine 554 (S-V-SP-E-A).

  • Positive control
    • extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours),

Properties

Applications

Our Abpromise guarantee covers the use of ab55228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 117 kDa).

Target

Anti-Hormone sensitive lipase (phospho S855) antibody images

  • All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228) at 1/500 dilution

    Lane 1 : extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours).
    Lane 2 : extracts from HeLa cells treated with Adriamycin (0.5ng/ml, 24hours), and treated with the immunising phosphopeptide.

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 117 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References for Anti-Hormone sensitive lipase (phospho S855) antibody (ab55228)

ab55228 has not yet been referenced specifically in any publications.

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