Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] (ab76492)

Overview

  • Product nameAnti-Hormone sensitive lipase (phospho S855) antibody [EPR2330]
    See all Hormone sensitive lipase primary antibodies
  • Description
    Rabbit monoclonal [EPR2330] to Hormone sensitive lipase (phospho S855)
  • SpecificityDetects HSL/LIPE only when phosphorylated on Serine 855
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Phospho-specific peptide corresponding to residues surrounding Serine 855 of Human HSL/LIPE

  • Positive control
    • Human skeletal muscle lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab76492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 - 1/5000. Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2).

    Is unsuitable for ICC/IF, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIn adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
    • PathwayGlycerolipid metabolism; triacylglycerol degradation.
    • Sequence similaritiesBelongs to the 'GDXG' lipolytic enzyme family.
    • Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Hormone sensitive lipase antibody
      • Hormone sensitive lipase testicular isoform antibody
      • Hormone-sensitive lipase antibody
      • HSL antibody
      • LHS antibody
      • Lipase hormone sensitive antibody
      • LIPE antibody
      • LIPS_HUMAN antibody
      see all

    Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] images

    • All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution

      Lane 1 : Human skeletal muscle lysate, untreated
      Lane 2 : Human skeletal muscle lysate, treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 117 , 83 kDa
      Observed band size : 83 kDa (why is the actual band size different from the predicted?)

    References for Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] (ab76492)

    ab76492 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.

    The Serine 855 of human HSL is equivalent to Serine 559 of mouse HSL, and this would recognize the Serine 559 of mouse HSL. The orthologous residue for isoform 2 of the rat HSL would be Serine 565, and this antibo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"