Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
Application notesELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 0.3 µg/ml. Predicted molecular weight: 282 kDa.
Preliminary experiments gave an approx 50 kDa band in human skin lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 282 kDa according to NP_001009931.1. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.
RelevanceHornerin has similar structural features to those of profilaggrin, an essential protein for keratinization of epidermal tissues. Hornerin may play a role in cornification.
Cellular localizationCytoplasmic granule. Note: Found in keratohyalin granules in the granular cells of the epidermis