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|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam’s IgM Horse ELISA kit (ab190532) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgM in horse serum and plasma.
In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|IgM Horse Antibody coated microwells||1 unit|
|IgM Horse Calibrator (lyophilized)||1 vial|
|IgM Horse HRP Conjugate||1 vial|
|Stop Solution||1 x 12ml|
|Chromogen Substrate Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab190532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab190532 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"