Overview

  • Product nameAnti-HOXA10 antibody
    See all HOXA10 primary antibodies
  • Description
    Rabbit polyclonal to HOXA10
  • Specificityab23392 recognises HoxA10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: CQGNSKGENA ANWLTAKSGR KKRCPYTKHQ TLELEKEFLF NMYLTRERRL, corresponding to N terminal amino acids 2-51 of Human HOXA10

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab23392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5'-AA[AT]TTTTATTAC-3'.
  • Sequence similaritiesBelongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centaurin alpha 1 like antibody
    • Homeo box A10 antibody
    • Homeobox A10 antibody
    • Homeobox protein 1H antibody
    • Homeobox protein Hox A10 antibody
    • Homeobox protein Hox-1.8 antibody
    • Homeobox protein Hox-1H antibody
    • Homeobox protein Hox-A10 antibody
    • Homeobox protein HOXA10 antibody
    • HOX 1 antibody
    • HOX 1.8 antibody
    • HOX 1H antibody
    • HOX A10 antibody
    • HOX1 antibody
    • HOX1.8 antibody
    • HOX1H antibody
    • HOXA 10 antibody
    • HOXA10 antibody
    • HOXA10 protein antibody
    • HXA10_HUMAN antibody
    • MGC12859 antibody
    • PL antibody
    see all

Anti-HOXA10 antibody images

  • Anti-HOXA10 antibody (ab23392) at 0.4 µg/ml + Jurkat cell lysate with 5% skim milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-HOXA10 antibody (ab23392)

ab23392 has not yet been referenced specifically in any publications.

Product Wall

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask y...

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Thank you for your reply.

As we do not have any other antibodies in our catalogue that we guarantee (or predict) to work in rat tissue, then I think the best way forward is to refund the cost of the antibody. To do so, could you please provid...

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Thank you for your reply.

I agree, there is a difference between the signal you are getting with the antibody, compared to the signal that was seen when it was tested in the lab.

I still think that the antibody is detecting correct ...

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Thank you for calling Abcam earlier today.

Attached is the western blot image that we talked about on the telephone and I have highlighted the 3 bands that the antibody is detecting. Are these the same size of bands that you are seeing?
...

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Thank you for your enquiry. We have only tested the antibody in Western blotting and so far we do not have any feedback from customers on using the antibody in IHC. You could test the antibody in nuclear vs cytoplasmic extracts for example in Weste...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"