ICC/IF image of ab28699 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab28699, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.However, this Fast-Track antibody is not yet fully characterised.This image represents inconclusive preliminary data.
References for Anti-HOXA11 antibody (ab28699)
This product has been referenced in:
Connell KA et al. HOXA11 promotes fibroblast proliferation and regulates p53 in uterosacral ligaments. Reprod Sci16:694-700 (2009).
Read more (PubMed: 19372592) »
Connell KA et al. HOXA11 is critical for development and maintenance of uterosacral ligaments and deficient in pelvic prolapse. J Clin Invest118:1050-5 (2008).
Read more (PubMed: 18274672) »