The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa).
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Tissue specificityEmbryonic nervous system.
Sequence similaritiesBelongs to the Antp homeobox family. Deformed subfamily. Contains 1 homeobox DNA-binding domain.