Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXA4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HOXA4.

    (Peptide available as ab32311.)

  • Positive control
    • HESCS differentiated neuroectoderm lysate at day 10; HESCS differentiated neuroectoderm lysate at day 30.

Properties

Applications

Our Abpromise guarantee covers the use of ab26097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37-39 kDa (predicted molecular weight: 34 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
    • Tissue specificity
      Embryonic nervous system.
    • Sequence similarities
      Belongs to the Antp homeobox family. Deformed subfamily.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dfd like protein antibody
      • Homeo box A4 antibody
      • Homeobox A4 antibody
      • Homeobox protein Hox-1.4 antibody
      • Homeobox protein Hox-1D antibody
      • Homeobox protein Hox-A4 antibody
      • Hox 1.4 like protein antibody
      • hox-1.4 antibody
      • hox-1d antibody
      • HOX1 antibody
      • HOX1D antibody
      • HOXA4 antibody
      • HXA4_HUMAN antibody
      see all

    Images

    • Lane 1 : Marker
      Lanes 2-4 : Anti-HOXA4 antibody (ab26097) at 1 µg/ml

      Lane 2 : Neuroectoderm (HESCS differentiated) Lysate - Day 10 at 20 µg

      Lane 3 : Neuroectoderm (HESCS differentiated) Lysate - Day 30 at 20 µg

      Lane 4 : Human Skeletal Muscle Normal Tissue Lysate at 20 µg


      Secondary
      Lanes 2-4 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 30 kDa (why is the actual band size different from the predicted?)



      Higher migrating bands observed in Neuroectoderm (HESCS differentiated) Lysate - Day 10 may represent splice variants of HoxA4.  The immunogen is specific for HoxA4 in NCBI blast and shows no similarity to other HOX proteins.

    References

    This product has been referenced in:
    • Klausen C & Auersperg N HOXA4 protein levels and localization in the aorta and in human abdominal aortic aneurysms. BMC Physiol 11:18 (2011). WB ; Human . Read more (PubMed: 22168796) »
    • Lillvis JH  et al. Regional expression of HOXA4 along the aorta and its potential role in human abdominal aortic aneurysms. BMC Physiol 11:9 (2011). WB, IHC . Read more (PubMed: 21627813) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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