The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37-39 kDa (predicted molecular weight: 34 kDa).
Application notesIs unsuitable for ICC/IF or IHC-P.
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Tissue specificityEmbryonic nervous system.
Sequence similaritiesBelongs to the Antp homeobox family. Deformed subfamily. Contains 1 homeobox DNA-binding domain.
Lane 1 : Marker Lanes 2 - 4 : Anti-HOXA4 antibody (ab26097) at 1 µg/ml
Lane 1 : As above Lane 2 : Neuroectoderm (HESCS differentiated) Lysate - Day 10 at 20 µg
Lane 3 : Neuroectoderm (HESCS differentiated) Lysate - Day 30 at 20 µg
Lane 4 : Human Skeletal Muscle Normal Tissue Lysate at 20 µg