The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37-39 kDa (predicted molecular weight: 34 kDa).
Is unsuitable for ICC/IF or IHC-P.
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Embryonic nervous system.
Belongs to the Antp homeobox family. Deformed subfamily. Contains 1 homeobox DNA-binding domain.
Higher migrating bands observed in Neuroectoderm (HESCS differentiated) Lysate - Day 10 may represent splice variants of HoxA4. The immunogen is specific for HoxA4 in NCBI blast and shows no similarity to other HOX proteins.