Overview

  • Product nameAnti-HOXB13 antibody
    See all HOXB13 primary antibodies
  • Description
    Rabbit polyclonal to HOXB13
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pufferfish (Fugu), Zebrafish, Japanese ricefish
  • Immunogen

    Synthetic peptide:

    EREYAANKFITKDKRRKI

    , corresponding to amino acids 232 - 249 of Human HOXB13

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 24189220
WB 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ELISA 1/100 - 1/1000.

Target

Anti-HOXB13 antibody images

  • All lanes : Anti-HOXB13 antibody (ab28575) at 1/5000 dilution

    Lane 1 : Hela cell lysate with no immunizing peptide
    Lane 2 : Hela cell lysate with immunizing peptide

    Lysates/proteins at 20 µg/ml per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References for Anti-HOXB13 antibody (ab28575)

This product has been referenced in:
  • Cantile M  et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . Read more (PubMed: 24189220) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (prostate tumour derived line PC3)
Loading amount 20 µg
Specification prostate tumour derived line PC3
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
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Verified customer

Submitted Jan 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"