Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXB13
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pufferfish, Zebrafish, Japanese ricefish
  • Immunogen

    Synthetic peptide:

    EREYAANKFITKDKRRKI

    , corresponding to amino acids 232 - 249 of Human HOXB13

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 24189220
WB 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ELISA 1/100 - 1/1000.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box B13 antibody
    • Homeobox B13 antibody
    • Homeobox protein Hox B13 antibody
    • Homeobox protein Hox-B13 antibody
    • Homeobox protein HoxB13 antibody
    • HOX B13 antibody
    • HOXB 13 antibody
    • HOXB13 antibody
    • HXB13_HUMAN antibody
    • PSGD antibody
    • PSGD protein antibody
    see all

Images

  • All lanes : Anti-HOXB13 antibody (ab28575) at 1/5000 dilution

    Lane 1 : Hela cell lysate with no immunizing peptide
    Lane 2 : Hela cell lysate with immunizing peptide

    Lysates/proteins at 20 µg/ml per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References

This product has been referenced in:
  • Aquino G  et al. Deregulation of paralogous 13 HOX genes in oral squamous cell carcinoma. Am J Cancer Res 5:3042-55 (2015). Read more (PubMed: 26693058) »
  • Cantile M  et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . Read more (PubMed: 24189220) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA buffer pH 8
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate tumour derived line PC3)
Loading amount
20 µg
Specification
prostate tumour derived line PC3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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