Anti-HOXB13 antibody [mAbcam53931] (ab53931)




Our Abpromise guarantee covers the use of ab53931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa corresponding to the tagged recombinant HOXB13 protein
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box B13 antibody
    • Homeobox B13 antibody
    • Homeobox protein Hox B13 antibody
    • Homeobox protein Hox-B13 antibody
    • Homeobox protein HoxB13 antibody
    • HOX B13 antibody
    • HOXB 13 antibody
    • HOXB13 antibody
    • HXB13_HUMAN antibody
    • PSGD antibody
    • PSGD protein antibody
    see all

Anti-HOXB13 antibody [mAbcam53931] images

  • Anti-HOXB13 antibody [mAbcam53931] (ab53931) at 5 µg/ml + HOXB13 Recombinant protein at 0.1 µg

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab53931 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53931, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 cells fixed in 4%PFA at 1ug/ml..
  • Overlay histogram showing HeLa cells stained with ab53931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53931, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-HOXB13 antibody [mAbcam53931] (ab53931)

ab53931 has not yet been referenced specifically in any publications.

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The antibody will detect a band at ˜37 kDa, the image given on the datasheet show 57 kDa which is becuase the GST tagged recombinant protein was used.

I hope this new info will also be helpful.

Thank you very much for contacting us. I am sorry to hear that you are experiencing problems with the antibody.

The protocol looks fine to me however I would like to draw your attention to the following details

- ab53931 is Mouse ...

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