Overview

  • Product name
    Anti-HOXB13 antibody [mAbcam53931]
    See all HOXB13 primary antibodies
  • Description
    Mouse monoclonal [mAbcam53931] to HOXB13
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human HOXB13.

    (Peptide available as ab57024.)

  • Positive control
    • This antibody gave a positive signal against the HOXB13 (Human) Recombinant Protein.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab53931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 25745994
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa corresponding to the tagged recombinant HOXB13 protein
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box B13 antibody
    • Homeobox B13 antibody
    • Homeobox protein Hox B13 antibody
    • Homeobox protein Hox-B13 antibody
    • Homeobox protein HoxB13 antibody
    • HOX B13 antibody
    • HOXB 13 antibody
    • HOXB13 antibody
    • HXB13_HUMAN antibody
    • PSGD antibody
    • PSGD protein antibody
    see all

Images

  • Anti-HOXB13 antibody [mAbcam53931] (ab53931) at 5 µg/ml + HOXB13 Recombinant protein at 0.1 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab53931 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53931, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 cells fixed in 4%PFA at 1ug/ml..
  • Overlay histogram showing HeLa cells stained with ab53931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53931, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Jiang Y  et al. Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene 34:6079-91 (2015). IHC-P ; Human . Read more (PubMed: 25745994) »

See 1 Publication for this product

Customer reviews and Q&As

The antibody will detect a band at ˜37 kDa, the image given on the datasheet show 57 kDa which is becuase the GST tagged recombinant protein was used.

I hope this new info will also be helpful.

Thank you very much for contacting us. I am sorry to hear that you are experiencing problems with the antibody.

The protocol looks fine to me however I would like to draw your attention to the following details

- ab53931 is Mouse ...

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