Overview

  • Product nameAnti-Hoxb3 antibody
    See all Hoxb3 primary antibodies
  • Description
    Rabbit polyclonal to Hoxb3
  • SpecificityA region of Human HOXB3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (SCMRPGLAPE PLSAPPGSPP PSAAPTSATS NSSNGGGPSK SGPPKCGPGT) of human Hoxb3 (NP_002137).

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 2G antibody
    • Homeo box B3 antibody
    • Homeobox B3 antibody
    • Homeobox protein Hox-2.7 antibody
    • Homeobox protein Hox-2G antibody
    • Homeobox protein Hox-B3 antibody
    • Homeobox protein HoxB3 antibody
    • Hox 2.7 antibody
    • HOX 2G antibody
    • HOX2 antibody
    • Hox2.7 antibody
    • HOX2G antibody
    • HOXB3 antibody
    • HXB3_HUMAN antibody
    see all

Anti-Hoxb3 antibody images

  • Anti-Hoxb3 antibody (ab82945) at 1 µg/ml (diluted in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-Hoxb3 antibody (ab82945)

ab82945 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"