Overview

  • Product nameAnti-Hoxb3 antibody
    See all Hoxb3 primary antibodies
  • Description
    Rabbit polyclonal to Hoxb3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide within residues: GPSKSGPPKC GPGTNSTLTK QIFPWMKESR QTSKLKNNSP GTAEGCGGGG , corresponding to N terminal amino acids 108-157 of Human Hoxb3 (NP_002137)

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 2G antibody
    • Homeo box B3 antibody
    • Homeobox B3 antibody
    • Homeobox protein Hox-2.7 antibody
    • Homeobox protein Hox-2G antibody
    • Homeobox protein Hox-B3 antibody
    • Homeobox protein HoxB3 antibody
    • Hox 2.7 antibody
    • HOX 2G antibody
    • HOX2 antibody
    • Hox2.7 antibody
    • HOX2G antibody
    • HOXB3 antibody
    • HXB3_HUMAN antibody
    see all

Anti-Hoxb3 antibody images

  • Anti-Hoxb3 antibody (ab83404) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

References for Anti-Hoxb3 antibody (ab83404)

ab83404 has not yet been referenced specifically in any publications.

Product Wall

I have processed your request to receive a refund for ab83404 which did not work in western blot. Your credit note number is ******.

Thank you for your reply and for providing the order details. ab843404 is covered under our Abpromsie and so is guaranteed to work in western blotting on human samples. As it is clearly not working as promised, then I can either send you a replacement ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"