Overview

  • Product nameAnti-HOXB4 antibody
    See all HOXB4 primary antibodies
  • Description
    Rabbit polyclonal to HOXB4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: MAMSSFLINS NYVDPKFPPC EEYSQSDYLP SDHSPGYYAG GQRRESSFQP , corresponding to amino acids 1-50 of Human HOXB4

  • Positive control
    • WB: Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab56049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family. Deformed subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 2F antibody
    • Homeo box B4 antibody
    • Homeobox 2F antibody
    • Homeobox B4 antibody
    • Homeobox protein Hox B4 antibody
    • Homeobox protein Hox-2.6 antibody
    • Homeobox protein Hox-2F antibody
    • Homeobox protein Hox-B4 antibody
    • Homeobox protein HoxB4 antibody
    • HOX 2 antibody
    • Hox 2.6 antibody
    • Hox 2F antibody
    • HOX B4 antibody
    • HOX2 antibody
    • Hox2.6 antibody
    • Hox2F antibody
    • HOXB 4 antibody
    • hoxb4 antibody
    • HXB4_HUMAN antibody
    see all

Anti-HOXB4 antibody images

  • Anti-HOXB4 antibody (ab56049) at 2.5 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-HOXB4 antibody (ab56049)

This product has been referenced in:
  • Billon N  et al. Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development. Genome Biol 11:R80 (2010). WB ; Mouse . Read more (PubMed: 20678241) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"