Overview

  • Product nameAnti-HOXB4 antibody [EP1919Y]
    See all HOXB4 primary antibodies
  • Description
    Rabbit monoclonal [EP1919Y] to HOXB4
  • Tested applicationsSuitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the middle region of human HOXB4.

  • Positive control
    • K562 cell lysate, human placental tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/40.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family. Deformed subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 2F antibody
    • Homeo box B4 antibody
    • Homeobox 2F antibody
    • Homeobox B4 antibody
    • Homeobox protein Hox B4 antibody
    • Homeobox protein Hox-2.6 antibody
    • Homeobox protein Hox-2F antibody
    • Homeobox protein Hox-B4 antibody
    • Homeobox protein HoxB4 antibody
    • HOX 2 antibody
    • Hox 2.6 antibody
    • Hox 2F antibody
    • HOX B4 antibody
    • HOX2 antibody
    • Hox2.6 antibody
    • Hox2F antibody
    • HOXB 4 antibody
    • hoxb4 antibody
    • HXB4_HUMAN antibody
    see all

Anti-HOXB4 antibody [EP1919Y] images

  • Anti-HOXB4 antibody [EP1919Y] (ab76093) at 1/500 dilution + K562 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human placental tissue, using ab76093, at 1/100 dilution.
  • Overlay histogram showing K562 cells stained with ab76093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76093, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-HOXB4 antibody [EP1919Y] (ab76093)

ab76093 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76093.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"