Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXB5
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 (DPAAMHTGSY GYNYNGMDLS VNRSSASSSH FGAVGESSRA FPAPAQEPRF) of Human HOXB5 (NP_002138).

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Tissue specificity
    Spinal cord.
  • Sequence similarities
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Embryo.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGNC:5116 antibody
    • HHO.C10 antibody
    • Homeo box 2A antibody
    • Homeo box B5 antibody
    • Homeobox 2A antibody
    • Homeobox B5 antibody
    • Homeobox protein HHO.C10 antibody
    • Homeobox protein Hox B5 antibody
    • Homeobox protein Hox-2A antibody
    • Homeobox protein Hox-B5 antibody
    • Homeobox protein HoxB5 antibody
    • Homeobox protein Hu-1 antibody
    • Homeobox2A antibody
    • HomeoboxB5 antibody
    • HOX 2 antibody
    • Hox 2.1 antibody
    • HOX 2A antibody
    • HOX B5 antibody
    • HOX2 antibody
    • Hox2.1 antibody
    • Hox2.1, mouse, homolog of antibody
    • HOX2A antibody
    • HOXB 5 antibody
    • Hoxb5 antibody
    • HU 1 antibody
    • HU1 antibody
    • HXB5_HUMAN antibody
    see all

Images

  • Anti-HOXB5 antibody (ab83025) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References

ab83025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83025.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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