Anti-HOXB6 antibody (ab26077)

Abreviews (1)Q&A (1)
Customer reviews and FAQs

Overview

  • Product name
    Anti-HOXB6 antibody
    See all HOXB6 primary antibodies
  • Description
    Rabbit polyclonal to HOXB6
  • Specificity
    ab26077 recognises Hox-B6 (HOXB6).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide derived from a region between residues 89-139 of human HOXB6.

    Read Abcam's proprietary immunogen policy

    .

  • Positive control
    • Fetal intestine lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab26077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • HGNC:5117 antibody
      • Homeo box 2B antibody
      • homeo box B6 antibody
      • Homeobox B6 antibody
      • Homeobox protein Hox B6 antibody
      • Homeobox protein Hox-2.2 antibody
      • Homeobox protein Hox-2B antibody
      • Homeobox protein Hox-B6 antibody
      • Homeobox protein Hu-2 antibody
      • Hox-2.2 antibody
      • HOX2 antibody
      • HOX2B antibody
      • hoxb6 antibody
      • HU-2 antibody
      • HXB6_HUMAN antibody
      see all

    Anti-HOXB6 antibody images

    • Western blot - HOXB6 antibody (ab26077)
      Western blot - HOXB6 antibody (ab26077)
      Anti-HOXB6 antibody (ab26077) at 1 µg/ml + Human A549 lung cancer cell line whole cell lysate

      Secondary
      Alkaline phosphatase conjugated goat anti-rabbit
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
      Additional bands at : 45 kDa (possible non-specific binding),52 kDa (possible non-specific binding).

      Exposure time : 25 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-HOXB6 antibody (ab26077)

    ab26077 has not yet been referenced specifically in any publications.

    Publishing research using ab26077? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab26077 Submit a question for ab26077

    2 Abreviews or Q&A

    10
    Votes: Highest First

    I'm looking for an antibody that i can use in an EMSA supershift assay. Computational models predict that various HOX proteins (including HOXA1, 2, 3, 6 and 7, and HOXB 5, 6, and 7, and HOXC 6 all might bind in that region. Do you have an antibody that...

    Read More

    We currently have over forty products against these HOX targets. These product will be specific to the HOX protein which they are raised against, whether A1, A2, A3 etc. We currently do not carry an anti-HOX antibody which will recognize a number of HO...

    Read More

    Western blot abreview for Anti-HOXB6 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 lung cancer cell line)
    Loading amount
    30 µg
    Specification
    A549 lung cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jan 26 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up