Anti-HOXB6 antibody (ab26077)

Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXB6
  • Specificity
    ab26077 recognises Hox-B6 (HOXB6).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide derived from a region between residues 89-139 of human HOXB6.

    .

  • Positive control
    • Fetal intestine lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab26077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGNC:5117 antibody
    • Homeo box 2B antibody
    • homeo box B6 antibody
    • Homeobox B6 antibody
    • Homeobox protein Hox B6 antibody
    • Homeobox protein Hox-2.2 antibody
    • Homeobox protein Hox-2B antibody
    • Homeobox protein Hox-B6 antibody
    • Homeobox protein Hu-2 antibody
    • Hox-2.2 antibody
    • HOX2 antibody
    • HOX2B antibody
    • hoxb6 antibody
    • HU-2 antibody
    • HXB6_HUMAN antibody
    see all

Images

  • Anti-HOXB6 antibody (ab26077) at 1 µg/ml + Human A549 lung cancer cell line whole cell lysate

    Secondary
    Alkaline phosphatase conjugated goat anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 45 kDa (possible non-specific binding),52 kDa (possible non-specific binding).

    Exposure time : 25 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References

ab26077 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We currently have over forty products against these HOX targets. These product will be specific to the HOX protein which they are raised against, whether A1, A2, A3 etc. We currently do not carry an anti-HOX antibody which will recognize a number of HO...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 lung cancer cell line)
Loading amount
30 µg
Specification
A549 lung cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

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Verified customer

Submitted Jan 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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