For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to HOXB8
Predicted to work with:
Mouse, Rat, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to Human HOXB8 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P17481
This antibody gave a positive signal in both Human Fetal Liver and Human Fetal Brain tissue lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.
Expressed in whole embryos and fetuses at 5-9 weeks from conception.
Information by UniProt
Homeobox B8 antibody
Homeobox protein Hox B8 antibody
Homeobox protein Hox-2.4 antibody
Western blot - Anti-HOXB8 antibody (ab125727)
All lanes :
Anti-HOXB8 antibody (ab125727) at 1 µg/ml
Lane 1 :
Fetal Liver (Human) Normal Tissue Lysate
Lane 2 :
Brain (Human) Tissue Lysate - fetal normal tissue
Lysates/proteins at 25 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
32 kDa (
why is the actual band size different from the predicted?
Additional bands at :
48 kDa (possible non-specific binding),52 kDa (possible non-specific binding).
Exposure time :
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab125727 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"