Overview

  • Product nameAnti-HOXB8 antibody
    See all HOXB8 primary antibodies
  • Description
    Rabbit polyclonal to HOXB8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human HOXB8 aa 200 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P17481

  • Positive control
    • This antibody gave a positive signal in both Human Fetal Liver and Human Fetal Brain tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab125727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox B8 antibody
    • Homeobox protein Hox B8 antibody
    • Homeobox protein Hox-2.4 antibody
    • Homeobox protein Hox-2D antibody
    • Homeobox protein Hox-B8 antibody
    • Homeobox protein HoxB8 antibody
    • HOX 2 antibody
    • Hox 2.4 antibody
    • HOX 2D antibody
    • HOX B8 antibody
    • HOX2 antibody
    • Hox2.4 antibody
    • HOX2D antibody
    • Hoxb8 antibody
    • HXB8_HUMAN antibody
    see all

Anti-HOXB8 antibody images

  • All lanes : Anti-HOXB8 antibody (ab125727) at 1 µg/ml

    Lane 1 : Fetal Liver (Human) Normal Tissue Lysate
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa (possible non-specific binding),52 kDa (possible non-specific binding).

    Exposure time : 16 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab125727 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-HOXB8 antibody (ab125727)

ab125727 has not yet been referenced specifically in any publications.

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