Overview

  • Product nameAnti-HOXB8 antibody
    See all HOXB8 primary antibodies
  • Description
    Rabbit polyclonal to HOXB8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Pig
  • Immunogen

    Synthetic peptide from within a region of N terminal amino acids 2-51 (SSYFVNSLFS KYKTGESLRP NYYDCGFAQD LGGRPTVVYG PSSGGSFQHP) of Human HOXB8 (NP_076921)

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox B8 antibody
    • Homeobox protein Hox B8 antibody
    • Homeobox protein Hox-2.4 antibody
    • Homeobox protein Hox-2D antibody
    • Homeobox protein Hox-B8 antibody
    • Homeobox protein HoxB8 antibody
    • HOX 2 antibody
    • Hox 2.4 antibody
    • HOX 2D antibody
    • HOX B8 antibody
    • HOX2 antibody
    • Hox2.4 antibody
    • HOX2D antibody
    • Hoxb8 antibody
    • HXB8_HUMAN antibody
    see all

Anti-HOXB8 antibody images

  • Anti-HOXB8 antibody (ab86610) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal liver lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-HOXB8 antibody (ab86610)

ab86610 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryo E8.5 sections)
Specification mouse embryo E8.5 sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step 1%BSA+5%donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"