• Product name
  • Description
    Rabbit polyclonal to HOXB9
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: SLPSVYHPYI QPQGVPPAES RYLRTWLEPA PRGEAAPGQG QAAVKAEPLL, corresponding to amino acids 73-122 of Human HOXB9

  • Positive control
    • HepG2 cell lysate, human heart tissue and human lung tissue



Our Abpromise guarantee covers the use of ab66765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.25 µg/ml. Detects a band of approximately 28, 32, 85 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.


  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 2E antibody
    • Homeo box B9 antibody
    • Homeobox B9 antibody
    • Homeobox protein Hox B9 antibody
    • Homeobox protein Hox-2.5 antibody
    • Homeobox protein Hox-2E antibody
    • Homeobox protein Hox-B9 antibody
    • Hox 2.5 antibody
    • Hox 2E antibody
    • HOX2 antibody
    • HOX2E antibody
    • Hoxb9 antibody
    • HXB9_HUMAN antibody
    • OTTHUMP00000216946 antibody
    • OTTHUMP00000243325 antibody
    see all


  • Anti-HOXB9 antibody (ab66765) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 30 kDa,85 kDa. We are unsure as to the identity of these extra bands.There are also three other bands < 23kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.


This product has been referenced in:
  • Wu SY  et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun 7:11169 (2016). WB, IHC-P ; Human . Read more (PubMed: 27041221) »
  • Oliveira-Costa JP  et al. Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells. Oncotarget 6:20902-20 (2015). IHC . Read more (PubMed: 26041877) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your reply and for your interest in ab66765. I will have the blocking peptide added to the catalog, and I'll let you know the catalog number once this is completed. Since ab66765 has not been tested in bovine or murine samples, if you wou...

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Thank you for contacting us with your questions. We have not tested this antibody yet in bovine or murine tissue, however the immunogen is 100% homologous to the bovine sequence so it will most likely work. The immunogen is only 78% homologous to mouse...

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Thank you for contacting us and please accept all our apologies for this error and delay. Indeed, we had a confusion in our notes in regards to the reconstitution of this antibody. I can confirm now, that the vial you have received has been rec...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (Spinal cord)
Spinal cord
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

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Verified customer

Submitted Oct 21 2009


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