Overview

  • Product nameAnti-HOXB9 antibody
    See all HOXB9 primary antibodies
  • Description
    Mouse monoclonal to HOXB9
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein: PLSPHASGSL PSVYHPYIQP QGVPPAESRY LRTWLEPAPR GEAAPGQGQA AVKAEPLLGA PGELLKQGTP EYSLETSAGR EAVLSNQRPG YGDNKICEG, corresponding to amino acids 65 - 163 of human HOXB9 (NP_076922) with a 26 kDa tag

  • Positive control
    • HepG2 cell lysate; immunogen

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 2E antibody
    • Homeo box B9 antibody
    • Homeobox B9 antibody
    • Homeobox protein Hox B9 antibody
    • Homeobox protein Hox-2.5 antibody
    • Homeobox protein Hox-2E antibody
    • Homeobox protein Hox-B9 antibody
    • Hox 2.5 antibody
    • Hox 2E antibody
    • HOX2 antibody
    • HOX2E antibody
    • Hoxb9 antibody
    • HXB9_HUMAN antibody
    • OTTHUMP00000216946 antibody
    • OTTHUMP00000243325 antibody
    see all

Anti-HOXB9 antibody images

  • Anti-HOXB9 antibody (ab77456) at 1/500 dilution + recombinant tagged HOXB9 protein (the immunogen) at 0.2 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 28 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 29 & 30 kDa. We are unsure as to the identity of these extra bands.
  • Anti-HOXB9 antibody (ab77456) at 1/500 dilution + HepG2 cell lysate at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)- HRP conjugate at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HOXB9 antibody (ab77456)

ab77456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"