Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXC11
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: SVLPQAFDRF FDNAYCGGGD PPAEPPCSGK GEAKGEPEAP PASGLASRAE, corresponding to internal sequence amino acids 145-194 of Human HOXC11

  • Positive control
    • transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/1000.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to a promoter element of the lactase-phlorizin hydrolase gene.
  • Sequence similarities
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box C11 antibody
    • Homeobox C11 antibody
    • Homeobox protein Hox C11 antibody
    • Homeobox protein Hox-3H antibody
    • Homeobox protein Hox-C11 antibody
    • HOX3H antibody
    • HOXC11 antibody
    • HXC11_HUMAN antibody
    • MGC4906 antibody
    see all

Images

  • Sample Type : Mouse lumbar spinal cord sections. Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti-rabbit-Cy3. Secondary Antibody Dilution : 1:800. Color/Signal Descriptions : Red: HOXC11. Gene Name : HOXC11.
  • Anti-HOXC11 antibody (ab66773) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%

References

ab66773 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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