Recombinant Anti-HoxC6 antibody [EPR7922(2)] (ab151575)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7922(2)] to HoxC6
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HoxC6 antibody [EPR7922(2)]
See all HoxC6 primary antibodies -
Description
Rabbit monoclonal [EPR7922(2)] to HoxC6 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human HoxC6 aa 1-100. The exact sequence is proprietary.
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Positive control
- HUVEC, PC3, MCF7 and Jurkat whole cell lysate (ab7899); MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7922(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
Target
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Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Sequence similarities
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3223 Human
- Entrez Gene: 15425 Mouse
- Entrez Gene: 252885 Rat
- Omim: 142972 Human
- SwissProt: P09630 Human
- SwissProt: P10629 Mouse
- Unigene: 549040 Human
- Unigene: 4444 Mouse
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Alternative names
- CP25 antibody
- HHO.C8 antibody
- Homeo box 3C antibody
see all
Images
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All lanes : Anti-HoxC6 antibody [EPR7922(2)] (ab151575) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : PC3 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa -
Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling HoxC6 with ab151575 at 1/100 dilution (red), compared to a Rabbit IgG negative control antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab151575 has been referenced in 7 publications.
- Huang H et al. HOXC6 impacts epithelial-mesenchymal transition and the immune microenvironment through gene transcription in gliomas. Cancer Cell Int 22:170 (2022). PubMed: 35488304
- Long X et al. HomeoboxC6 affects the apoptosis of human vascular endothelial cells and is involved in atherosclerosis. J Cell Physiol 236:1913-1925 (2021). PubMed: 32740941
- Xu J et al. LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression. Cell Prolif 53:e12823 (2020). PubMed: 32515533
- Shen LY et al. Targeting HOX/PBX dimer formation as a potential therapeutic option in esophageal squamous cell carcinoma. Cancer Sci 110:1735-1745 (2019). PubMed: 30844117
- Yan TF et al. Knockdown of HOXC6 inhibits glioma cell proliferation and induces cell cycle arrest by targeting WIF-1 in vitro and vivo. Pathol Res Pract 214:1818-1824 (2018). PubMed: 30228024
- Zhang F et al. HOXC6 gene silencing inhibits epithelial-mesenchymal transition and cell viability through the TGF-ß/smad signaling pathway in cervical carcinoma cells. Cancer Cell Int 18:204 (2018). PubMed: 30559605
- Shen LY et al. Increased HOXC6 expression predicts chemotherapy sensitivity in patients with esophageal squamous cell carcinoma. Oncol Lett 14:4835-4840 (2017). PubMed: 29085488