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Rabbit polyclonal to HOXC8
Predicted to work with:
Mouse, Sheep, Chicken, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HOXC8.
(Peptide available as
This antibody gave a positive signal in Mouse Spinal Cord and Mouse Brain Tissue Lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.
Information by UniProt
Homeo box 3A antibody
Homeo box 8 antibody
Homeobox 3A antibody
Western blot - Anti-HOXC8 antibody (ab79690)
All lanes :
Anti-HOXC8 antibody (ab79690) at 1 µg/ml
Lane 1 :
Spinal Cord (Mouse) Tissue Lysate
Lane 2 :
Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Immunocytochemistry/ Immunofluorescence - HOXC8 antibody (ab79690)
ICC/IF image of ab79690 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"