Overview

  • Product nameAnti-HOXC8 antibody
    See all HOXC8 primary antibodies
  • Description
    Rabbit polyclonal to HOXC8
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 of Human HOXC8 (

    HALVYGPGGSAPGFQHASHHVQDFFHHGTSGISNSGYQQNPCSLSCHGDA

    ) NP_073149

  • Positive control
    • Fetal muscle lysate (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab86236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 25157815
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 3A antibody
    • Homeo box 8 antibody
    • Homeobox 3A antibody
    • Homeobox C8 antibody
    • Homeobox protein Hox C8 antibody
    • Homeobox protein Hox-3A antibody
    • Homeobox protein Hox-C8 antibody
    • Homeobox protein HoxC8 antibody
    • Homeobox protein R4 antibody
    • Homolog of mouse Hox 3.1 antibody
    • HOX 3 antibody
    • Hox 3.1 mouse homolog of antibody
    • HOX 3A antibody
    • HOX C8 antibody
    • Hox-3.1 antibody
    • Hox-3A antibody
    • HOX3 antibody
    • HOX3A antibody
    • HOXC 8 antibody
    • Hoxc-8 antibody
    • HOXC8 antibody
    • HXC8_HUMAN antibody
    • M31 antibody
    see all

Anti-HOXC8 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling HOXC8 with ab86236 at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling HOXC8 with ab86236 at 5µg/ml.

  • Anti-HOXC8 antibody (ab86236) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal muscle lysate (Human) at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

References for Anti-HOXC8 antibody (ab86236)

This product has been referenced in:
  • Gouti M  et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . Read more (PubMed: 25157815) »
  • Mori M  et al. Essential Role for miR-196a in Brown Adipogenesis of White Fat Progenitor Cells. PLoS Biol 10:e1001314 (2012). WB ; Human . Read more (PubMed: 22545021) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Human Cell (differentiated embryonic stem cells)
Specification differentiated embryonic stem cells
Permeabilization Yes - PBS/0.1% Triton X100 (PBST)
Fixative Formaldehyde
Username

Dr. Anestis Tsakiridis

Verified customer

Submitted May 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"