Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXD10
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human HOXD10.

    (Peptide available as ab90993.)

  • Positive control
    • This antibody gave a positive signal in JEG3 whole cell lysate and in the following tissue lysates: Human ovary; Human testis; Human prostate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-Fr 1/16000.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Tissue specificity
    Strongly expressed in the adult male and female urogenital tracts.
  • Involvement in disease
    Defects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity.
  • Sequence similarities
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed in the developing limb buds.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI385591 antibody
    • AI874987 antibody
    • Homeo box 4D antibody
    • Homeo box D10 antibody
    • Homeobox D10 antibody
    • Homeobox protein Hox-4D antibody
    • Homeobox protein Hox-4E antibody
    • Homeobox protein Hox-D10 antibody
    • Hox 4.4 antibody
    • Hox 4.5 antibody
    • Hox 4D antibody
    • Hox 4E antibody
    • Hox 5.3 antibody
    • HOX4D antibody
    • HOX4E antibody
    • HOXD10 antibody
    • HXD10 antibody
    • HXD10_HUMAN antibody
    • OTTMUSP00000017845 antibody
    • RP23-313J15.9 antibody
    see all

Images

  • All lanes : Anti-HOXD10 antibody (ab76897) at 1 µg/ml

    Lane 1 : Human ovary tissue lysate - total protein (ab30222)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human prostate tissue lysate - total protein (ab30304)
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 146 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab76897 staining HOXD10 in Mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/16000 in PBS + 0.1% Triton + 0.1% BSA) for 18 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Zhou Y  et al. Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells. Oncol Lett 13:1143-1148 (2017). WB ; Human . Read more (PubMed: 28454226) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Specification
Spinal Cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

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Verified customer

Submitted Jul 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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