The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Tissue specificityStrongly expressed in the adult male and female urogenital tracts.
Involvement in diseaseDefects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity.
Sequence similaritiesBelongs to the Abd-B homeobox family. Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in the developing limb buds.
All lanes : Anti-HOXD10 antibody (ab76897) at 1 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222) Lane 2 : Human testis tissue lysate - total protein (ab30257) Lane 3 : Human prostate tissue lysate - total protein (ab30304) Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 38 kDa Observed band size : 38 kDa Additional bands at : 146 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunohistochemistry (Frozen sections) - Anti-HOXD10 antibody (ab76897)This image is courtesy of an anonymous Abreview
ab76897 staining HOXD10 in Mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/16000 in PBS + 0.1% Triton + 0.1% BSA) for 18 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.