Overview

  • Product nameAnti-HOXD11 antibody - ChIP Grade
    See all HOXD11 primary antibodies
  • Description
    Mouse monoclonal to HOXD11 - ChIP Grade
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Opossum
  • Immunogen

    Recombinant fragment with tag: MNDFDECGQS AASMYLPGCA YYVAPSDFAS KPSFLSQPSS CQMTFPYSSN LAPHVQPVRE VAFRDYGLER AKWPYR, corresponding to amino acids 1-77 of Human HOXD11

  • Positive control
    • Jurkate and Hela cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab60715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. The detection limit for recombinant tagged HOXD11 is approximately 0.1ng/ml as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 10 µg/ml.
ChIP Use at an assay dependent dilution.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box 4F antibody
    • Homeo box D11 antibody
    • Homeobox D11 antibody
    • Homeobox protein Hox-4F antibody
    • Homeobox protein Hox-D11 antibody
    • Hox-4F antibody
    • HOX4 antibody
    • HOX4F antibody
    • HOXD11 antibody
    • HXD11_HUMAN antibody
    see all

Anti-HOXD11 antibody - ChIP Grade images

  • Anti-HOXD11 antibody - ChIP Grade (ab60715) at 1 µg/ml + Jurkat Cell lysate at 25 µg

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HOXD11 antibody - ChIP Grade (ab60715)

This product has been referenced in:
  • Salsi V  et al. HOXD13 binds DNA replication origins to promote origin licensing and is inhibited by geminin. Mol Cell Biol 29:5775-88 (2009). ChIP ; Human . Read more (PubMed: 19703996) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"