• Product nameAnti-HOXD13 antibody
    See all HOXD13 primary antibodies
  • Description
    Rabbit polyclonal to HOXD13
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human HOXD13.

    (Peptide available as ab22466.)

  • Positive control
    • In Western Blot, this antibody gave a positive signal against following lysates: HOXD13 Recombinant Protein; Mouse stomach tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: DU145


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab19866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 36 kDa).
IHC-FoFr 1/100 - 1/300.
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.


  • RelevanceThis gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • HOXD13 antibody
    • BDE antibody
    • BDSD antibody
    • Homeo box 4I antibody
    • Homeo box D13 antibody
    • Homeo box protein Hox D13 antibody
    • Homeo box protein HoxD13 antibody
    • Homeobox 4I antibody
    • Homeobox D13 antibody
    • homeobox protein Hox 4I antibody
    • Homeobox protein Hox D13 antibody
    • Homeobox protein Hox-D13 antibody
    • Homeobox protein HoxD13 antibody
    • Homeobox4I antibody
    • HomeoboxD13 antibody
    • Hox 4I antibody
    • HOX D13 antibody
    • Hox-4.8 antibody
    • Hox4I antibody
    • HOXD 13 antibody
    • HoxD13 antibody
    • SPD antibody
    see all

Anti-HOXD13 antibody images

  • ICC/IF image of ab19866 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab19866 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab19866 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) lung. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
  • Anti-HOXD13 antibody (ab19866) at 1 µg/ml + HOXD13 (Human) Recombinant Protein at 0.2 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    ab19866 was tested on a recombinant GST tagged HOXD13 protein (Human) predicted to run at 65 kDa.

References for Anti-HOXD13 antibody (ab19866)

This product has been referenced in:
  • Cantile M  et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . Read more (PubMed: 24189220) »
  • Illig R  et al. Breaking the seals: efficient mRNA detection from human archival paraffin-embedded tissue. RNA 15:1588-96 (2009). IHC-P ; Human . Read more (PubMed: 19549718) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Anorectal region)
Specification Anorectal region
Fixative Paraformaldehyde
Antigen retrieval step Other
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10%

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Submitted Feb 16 2007