- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-HOXD13 antibody
See all HOXD13 primary antibodies -
DescriptionRabbit polyclonal to HOXD13
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Human
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Immunogen
Three synthetic peptides (human) conjugated to KLH.
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Positive control
- Induced culture of E. coli (one shot Top10 competent cells).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesCrude serum, final bleed.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab86015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| WB |
Application notes
ELISA: Use at an assay dependent dilution. A minimum titer of 1/12000 is determined for one of the three peptides.
WB: 1/1000. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceThis gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 3239 Human
- Entrez Gene: 15433 Mouse
- Omim: 142989 Human
- SwissProt: P35453 Human
- SwissProt: P70217 Mouse
- Unigene: 152414 Human
- Unigene: 57227 Mouse
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Alternative names
- HOXD13 antibody
- BDE antibody
- BDSD antibody
see all
Images
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Western blot - HOXD13 antibody (ab86015)All lanes : Anti-HOXD13 antibody (ab86015) at 1/1000 dilution
Lane 1 : Uninduced culture
Lane 2 : Induced culture of E.coli (one shot Top10 competent cells)
Secondary
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Plasmid name: pBAD-DEST49
Molecular weight of HOXD13: 50.1kDa (36.1kDa + another 14kDa for the tag).
Protocols
References
ab86015 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"