Overview

  • Product nameAnti-HOXD3 antibody
    See all HOXD3 primary antibodies
  • Description
    Rabbit polyclonal to HOXD3
  • Specificityab22840 reacts with HOXD3 (homeobox D3).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    AELNGSCMRPGTGNSQGGGGGSQPPGLNSEQQPPQPPPPPPTLPPSSPTN PGGGV

    , corresponding to amino acids 84-138 of Human HOXD3.

  • Positive control
    • Jurkat cell lysate, Human kidney and human muscle.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similaritiesBelongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box D3 antibody
    • Homeobox D3 antibody
    • Homeobox protein Hox D3 antibody
    • Homeobox protein Hox-4A antibody
    • Homeobox protein Hox-D3 antibody
    • Homeobox protein HoxD3 antibody
    • Homeodomain protein antibody
    • HOX 1D antibody
    • HOX 4 antibody
    • Hox 4.1 antibody
    • Hox 4A antibody
    • HOX D3 antibody
    • Hox-4.1 mouse homolog of antibody
    • HOX1D antibody
    • HOX4 antibody
    • Hox4.1 antibody
    • HOX4A antibody
    • HOXD 3 antibody
    • HOXD3 antibody
    • HXD3_HUMAN antibody
    • MGC10470 antibody
    see all

Anti-HOXD3 antibody images

  • Anti-HOXD3 antibody (ab22840) at 1 µg/ml + Jurkat cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ab22840, at a 1/125-250 dilution, staining human HOXD3 in human kidney by Immunohistochemistry, Paraffin embedded tissue. Positive labeled cells, marked with arrows, are epithelial cells of Renal Tubule. Magnification 400 x.
  • ab22840, at a 1/125-250 dilution, staining human HOXD3 in human muscle by Immunohistochemistry, Paraffin embedded tissue. Positive labeled cells, marked with arrows, are skeletal muscle cells. Magnification 400 x.

References for Anti-HOXD3 antibody (ab22840)

ab22840 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"