Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXD3
  • Specificity
    ab22840 reacts with HOXD3 (homeobox D3).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    AELNGSCMRPGTGNSQGGGGGSQPPGLNSEQQPPQPPPPPPTLPPSSPTN PGGGV

    , corresponding to amino acids 84-138 of Human HOXD3.

  • Positive control
    • Jurkat cell lysate, Human kidney and human muscle.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box D3 antibody
    • Homeobox D3 antibody
    • Homeobox protein Hox D3 antibody
    • Homeobox protein Hox-4A antibody
    • Homeobox protein Hox-D3 antibody
    • Homeobox protein HoxD3 antibody
    • Homeodomain protein antibody
    • HOX 1D antibody
    • HOX 4 antibody
    • Hox 4.1 antibody
    • Hox 4A antibody
    • HOX D3 antibody
    • Hox-4.1 mouse homolog of antibody
    • HOX1D antibody
    • HOX4 antibody
    • Hox4.1 antibody
    • HOX4A antibody
    • HOXD 3 antibody
    • HOXD3 antibody
    • HXD3_HUMAN antibody
    • MGC10470 antibody
    see all

Images

  • Anti-HOXD3 antibody (ab22840) at 1 µg/ml + Jurkat cell lysate.

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ab22840, at a 1/125-250 dilution, staining human HOXD3 in human kidney by Immunohistochemistry, Paraffin embedded tissue. Positive labeled cells, marked with arrows, are epithelial cells of Renal Tubule. Magnification 400 x.
  • ab22840, at a 1/125-250 dilution, staining human HOXD3 in human muscle by Immunohistochemistry, Paraffin embedded tissue. Positive labeled cells, marked with arrows, are skeletal muscle cells. Magnification 400 x.

References

ab22840 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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