Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab50915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities
    Belongs to the Antp homeobox family. Deformed subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HHO.C13 antibody
    • homeo box D4 antibody
    • homeobox D4 antibody
    • Homeobox protein HHO.C13 antibody
    • Homeobox protein Hox-4B antibody
    • Homeobox protein Hox-5.1 antibody
    • Homeobox protein Hox-D4 antibody
    • Hox-4.2 antibody
    • Hox-4.2, mouse, homolog of homeo box X antibody
    • HOX-5.1 antibody
    • HOX4 antibody
    • HOX4B antibody
    • HOXD4 antibody
    • HXD4_HUMAN antibody
    see all

Images

  • Anti-HOXD4 antibody (ab50915) at 0.5 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References

ab50915 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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