Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXD9
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (GPSPGPSGPA NGRHYGIKPE TRAAPAPATA ASTTSSSSTS LSSSSKRTEC) of Human HOXD9 (NP_055028).

  • Positive control
    • Human fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 24651450

Target

Images

  • Anti-HOXD9 antibody (ab90260) at 1 µg/ml + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa

References

This product has been referenced in:
  • Toshner M  et al. Transcript analysis reveals a specific HOX signature associated with positional identity of human endothelial cells. PLoS One 9:e91334 (2014). IHC-P ; Human . Read more (PubMed: 24651450) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2014

Thank you for the details of your samples and protocol. This is strong evidence that the antibody cannot detect mouse HOXD9. The "predicted" reactivity with the mouse protein is based on homology with the immunogen amino acid sequence, but the shared i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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