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Western blot - HP1 alpha antibody (ab16482)
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Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody (ab16482)
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Product Name
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HP1 alpha antibody
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See all HP1 alpha antibodies (6)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to HP1 alpha
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Immunogen
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Synthetic peptide, conjugated to KLH, correlating to aa from within regions 1-40 and 40-90 (separated with a GG linker) of Human HP1 alpha.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, ChHm
(Due to sequence homology)
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Specificity
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This antibody detects a strong band at 26kDa representing HP1 alpha.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
Previous customer feedback suggested that this antibody did not work in ICC/IF in interphase HeLa and SK-N-SH cells as no specific binding was detected(see Abreivews). More recent testing gave a positive result with this antibody. This difference may be due to different batches being tested. We welcome any further feedback about this antibody
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Chromatin
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
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Relevance
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HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane.
HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required
for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.025% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HP1 alpha antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HP1 alpha antibody images, references, products related to ab16482 and other tools.
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HP1 alpha antibody images:
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 Western blot - HP1 alpha antibody (ab16482)
Western Blot
Lane 1 - 6 : HP1 alpha antibody (ab16482) at 1/500
Lanes 4 - 6 : HP1 alpha peptide (ab4718) at 1 ug/ml
Lane 1 : HeLa Whole cell lysate
Lane 2 : HeLa Nuclear cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa Whole cell lysate
Lane 5 : HeLa Nuclear cell lysate
Lane 6 : A431 cell lysate
Lysates at 20 ug/lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 26kD
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Immunocytochemistry/ Immunofluorescence - HP1 alpha antibody (ab16482)
ICC/IF image of ab16482 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16482, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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References for HP1 alpha antibody (ab16482)
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This product has been used in:
Luo SW et al. Regulation of heterochromatin remodelling and myogenin expression during muscle differentiation by FAK interaction with MBD2. EMBO J 28:2568-82 (2009). ICC/IF; Mouse. PubMed: 19661918
If you publish research using ab16482 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HP1 alpha
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HP1 alpha antibody - more information
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Customer reviews (feedback) regarding HP1 alpha antibody |
Customer FAQs regarding HP1 alpha antibody |
Protocols for HP1 alpha antibody |
Price and availability of products related to HP1 alpha antibody |
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HP1 alpha antibody - related products:
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HP1 alpha antibody - other tools:
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Contact Abcam with a Technical Enquiry about HP1 alpha antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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