Anti-HP1 alpha antibody - ChIP Grade (ab77256)

Overview

  • Product nameAnti-HP1 alpha antibody - ChIP Grade
    See all HP1 alpha primary antibodies
  • Description
    Goat polyclonal to HP1 alpha - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, IP, IHC-P, ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Rhesus monkey, Chinese Hamster
  • Immunogen

    Synthetic peptide:

    C-NKRKSNFSNSADDIK

    , corresponding to internal sequence amino acids 88-102 of Human HP1 alpha (NP_036249.1).

  • Positive control
    • Human fibroblast cell lines A431 and HeLa cell lysates. Human uterus tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab77256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 3 µg/ml.
ChIP Use at an assay dependent dilution. PubMed: 20663865
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • FunctionComponent of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similaritiesContains 2 chromo domains.
  • Post-translational
    modifications
    Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    Ubiquitinated.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen p25 antibody
    • CBX5 antibody
    • CBX5_HUMAN antibody
    • CG8409 antibody
    • Chromobox 5 antibody
    • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
    • Chromobox homolog 5 antibody
    • Chromobox protein homolog 5 antibody
    • Epididymis luminal protein 25 antibody
    • HEL25 antibody
    • Heterochromatin protein 1 alpha antibody
    • Heterochromatin protein 1 antibody
    • Heterochromatin protein 1 homolog alpha antibody
    • HP1 alpha antibody
    • HP1 alpha homolog antibody
    • HP1 antibody
    • HP1A antibody
    • HP1Hs alpha antibody
    • Su(var)205 antibody
    see all

Anti-HP1 alpha antibody - ChIP Grade images

  • Anti-HP1 alpha antibody - ChIP Grade (ab77256) at 0.03 µg/ml + HeLa lysate in RIPA buffer at 35 µg

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • ab77256 at 2.5µg/ml staining HP1 alpha in human uterus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

  • ab77256 staining HP1 alpha in Mouse 3T3 fibroblasts by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 0.2% Triton X-100 in PBS. The primary antibody was diluted 1/100 in PBS + Tween 1:1000 and incubated with the sample for 30 minutes. The secondary antibody was Cy2®-conjugated Donkey anti-Goat polyclonal, diluted 1/100.

    See Abreview

References for Anti-HP1 alpha antibody - ChIP Grade (ab77256)

This product has been referenced in:
  • González-Barrios R  et al. Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells. Cell Div 9:6 (2014). Human . Read more (PubMed: 25729403) »
  • Chan KM & Zhang Z Leucine-rich Repeat and WD Repeat-containing Protein 1 Is Recruited to Pericentric Heterochromatin by Trimethylated Lysine 9 of Histone H3 and Maintains Heterochromatin Silencing. J Biol Chem 287:15024-33 (2012). WB . Read more (PubMed: 22427655) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (B Cells)
Negative control Isotype IgG
Specification B Cells
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control Specific Gene Promoter
Username

Abcam user community

Verified customer

Submitted Nov 28 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Ahmar Aziz

Verified customer

Submitted May 03 2016

Application Western blot
Sample Rat Cell lysate - whole cell (Epithelial cells)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 75 µg
Specification Epithelial cells
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa, RPE-1)
Specification HeLa, RPE-1
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2013

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (HEK-293)
Total protein in input 1000 µg
Specification HEK-293
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Mar 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HEK-293)
Loading amount 200000 cells
Specification HEK-293
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Benny Garfinkel

Verified customer

Submitted Feb 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Jurkat)
Specification Jurkat
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100 in PBS
Username

Mr. Benny Garfinkel

Verified customer

Submitted Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (3T3 fibroblasts)
Specification 3T3 fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100 in PBS
Username

Mr. Benny Garfinkel

Verified customer

Submitted Jan 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"