Overview

  • Product nameAnti-HP1 gamma antibody
    See all HP1 gamma primary antibodies
  • Description
    Goat polyclonal to HP1 gamma
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    EAFLNSQKAGKEKD

    conjugated to KLH, corresponding to internal sequence amino acids 74-87 of Human HP1 gamma

  • Positive control
    • Human placenta tissue, A431 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similaritiesContains 2 chromo domains.
  • Post-translational
    modifications
    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Anti-HP1 gamma antibody images

  • Human placenta (formalin fixed, paraffin embedded) stained with ab115221 at 2.5 ug/ml followed by biotinylated anti Goat IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
  • Anti-HP1 gamma antibody (ab115221) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 21 kDa

References for Anti-HP1 gamma antibody (ab115221)

ab115221 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"