Anti-HP1 gamma/CBX3 antibody (ab227478)
Key features and details
- Rabbit polyclonal to HP1 gamma/CBX3
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HP1 gamma/CBX3 antibody
See all HP1 gamma/CBX3 primary antibodies -
Description
Rabbit polyclonal to HP1 gamma/CBX3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Rhesus monkey -
Immunogen
Recombinant fragment within Human HP1 gamma/CBX3 (internal sequence). The exact sequence is proprietary.
Database link: Q13185 -
Positive control
- WB: HeLa cell extracts; HepG2 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Mouse duodenum and brain tissues; TOV-21G xenograft tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 21 kDa.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 21 kDa. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsPhosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. -
Cellular localization
Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 395355 Chicken
- Entrez Gene: 539788 Cow
- Entrez Gene: 11335 Human
- Entrez Gene: 12417 Mouse
- Entrez Gene: 297093 Rat
- Entrez Gene: 706978 Rhesus monkey
- Entrez Gene: 399208 Xenopus laevis
- Omim: 604477 Human
see all -
Alternative names
- CBX 3 antibody
- CBX3 antibody
- CBX3_HUMAN antibody
see all
Images
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Anti-HP1 gamma/CBX3 antibody (ab227478) at 1/20000 dilution + WT HeLa cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa -
All lanes : Anti-HP1 gamma/CBX3 antibody (ab227478) at 1/20000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : HP1 gamma knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
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HP1 gamma/CBX3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab227478. Western blot was performed from the immunoprecipitate using ab227478 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell lysate 40 μg.
Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.
Lane 3: ab227478 IP in HeLa whole cell lysate.
15% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for HP1 gamma/CBX3 using ab227478 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for HP1 gamma/CBX3 using ab227478 at 1/500 dilution in immunohistochemical analysis.
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Anti-HP1 gamma/CBX3 antibody (ab227478) at 1/10000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 21 kDa12% SDS-PAGE gel.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 gamma/CBX3 (green) using ab227478 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-alpha tubulin antibody at 1/2000 dilution (red).
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Paraffin-embedded TOV-21G (human) xenograft tissue stained for HP1 gamma/CBX3 using ab227478 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227478 has not yet been referenced specifically in any publications.