Overview

  • Product nameAnti-HP1 gamma antibody
    See all HP1 gamma primary antibodies
  • Description
    Goat polyclonal to HP1 gamma
  • SpecificityNo cross-reactivity is expected with HP1-alpha and HP1-beta.
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Internal synthetic peptide:

    (C)EAFLNSQKAGKEKD

    (Human)

  • Positive control
    • Cell lysates of A431, HeLa and HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab40827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 3 µg/ml.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similaritiesContains 2 chromo domains.
  • Post-translational
    modifications
    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Anti-HP1 gamma antibody images

  • Anti-HP1 gamma antibody (ab40827) at 0.3 µg/ml + A431 cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • ab40827 at 2.5µg/ml staining HP1 gamma in human placenta tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

References for Anti-HP1 gamma antibody (ab40827)

This product has been referenced in:
  • Teperek M  et al. Sperm and spermatids contain different proteins and bind distinct egg factors. Int J Mol Sci 15:16719-40 (2014). WB ; Frog . Read more (PubMed: 25244019) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Whole tadpole lysate)
Loading amount 1e+006 cells
Specification Whole tadpole lysate
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"