The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: Use at a concentration of 1 µg/ml for enriched sources (e.g.
Immunoprecipitates). Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Not recommended for crude preparations.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similaritiesContains 2 chromo domains.
Post-translational modificationsPhosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
Detection of HP1 gamma by Western Blot of Immunprecipitate.
ab72042 at 1µg/ml staining HP1 gamma in HeLa whole cell lysate immunoprecipitated using ab72042 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-HP1 gamma antibody (ab72042)
has not yet been referenced specifically in any publications.
Publishing research using ab72042? Please let us know so that we can cite the reference in this datasheet.
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