Overview

  • Product nameAnti-HP1 gamma antibody
    See all HP1 gamma primary antibodies
  • Description
    Rabbit polyclonal to HP1 gamma
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 75 and 125 of human HP1 gamma (NP_009207.2).

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: Use at a concentration of 1 µg/ml for enriched sources (e.g.
    Immunoprecipitates). Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Not recommended for crude preparations.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    • Sequence similaritiesContains 2 chromo domains.
    • Post-translational
      modifications
      Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    • Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • CBX 3 antibody
      • CBX3 antibody
      • CBX3_HUMAN antibody
      • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
      • Chromobox homolog 3 antibody
      • Chromobox protein homolog 3 antibody
      • GAMMA antibody
      • HECH antibody
      • Heterochromatin like protein 1 antibody
      • Heterochromatin protein 1 homolog gamma antibody
      • Heterochromatin protein HP1 gamma antibody
      • HP1 gamma antibody
      • HP1 gamma homolog antibody
      • HP1Hs gamma antibody
      • Modifier 2 protein antibody
      see all

    Anti-HP1 gamma antibody images

    • Detection of HP1 gamma by Western Blot of Immunprecipitate.
      ab72042 at 1µg/ml staining HP1 gamma in HeLa whole cell lysate immunoprecipitated using ab72042 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    References for Anti-HP1 gamma antibody (ab72042)

    ab72042 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Total protein in input 1e+006 cells
    Specification HEK293 cells
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Aug 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"