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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse HP1 gamma.
Our Abpromise guarantee covers the use of ab10480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 gamma knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10480 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10480 was shown to recognize HP1 gamma when HP1 gamma knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma knockout samples were subjected to SDS-PAGE. ab10480 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.