Validated using a knockout cell line

Anti-HP1 gamma antibody [EPR10465(B)] (ab154871)


  • Product name
    Anti-HP1 gamma antibody [EPR10465(B)]
    See all HP1 gamma primary antibodies
  • Description
    Rabbit monoclonal [EPR10465(B)] to HP1 gamma
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HP1 gamma (Q13185).

  • Positive control
    • NIH/3T3, HeLa, Molt-4 and HepG2 cell lysates; Human breast carcinoma tissue; Human normal breast tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab154871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    • Sequence similarities
      Contains 2 chromo domains.
    • Post-translational
      Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    • Cellular localization
      Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • CBX 3 antibody
      • CBX3 antibody
      • CBX3_HUMAN antibody
      • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
      • Chromobox homolog 3 antibody
      • Chromobox protein homolog 3 antibody
      • GAMMA antibody
      • HECH antibody
      • Heterochromatin like protein 1 antibody
      • Heterochromatin protein 1 homolog gamma antibody
      • Heterochromatin protein HP1 gamma antibody
      • HP1 gamma antibody
      • HP1 gamma homolog antibody
      • HP1Hs gamma antibody
      • Modifier 2 protein antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HP1 gamma knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH3T3 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab154871 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab154871was shown to recognize HP1 gamma when HP1 gamma knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma knockout samples were subjected to SDS-PAGE. ab154871 and ab8245 (loading control to GAPDH) were both diluted to 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing HeLa cells stained with ab154871 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154871, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-HP1 gamma antibody [EPR10465(B)] (ab154871) at 1/1000 dilution

      Lane 1 : NIH/3T3 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HP1 gamma with ab154871 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling HP1 gamma with ab154871 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154871 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154871 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154871 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154871 showing +ve staining.


    This product has been referenced in:
    • Yang G  et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). ChIP . Read more (PubMed: 28361889) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154871.
    Please use the links above to contact us or submit feedback about this product.


    Sign up