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Overview
Product name
Anti-HP1 gamma (unmodified S93) antibody
See all HP1 gamma products (11) ...
Description
Rabbit polyclonal to HP1 gamma (unmodified S93)
Specificity
Detects endogenous levels of total HP1 gamma protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized non-phosphopeptide derived from human HP1 gamma around the phosphorylation site of serine 93 (RKSPLS).
Positive control
293 nuclear lysate
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Ca2+ and Mg2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - HP1 gamma antibody (ab79304)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 21 kDa.
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similarities
Contains 2 chromo domains.
Post-translational
modifications
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localization
Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
Target information above from: UniProt accessionQ13185
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CBX 3 antibody
- CBX3 antibody
- CBX3_HUMAN antibody
see all
Database links
- Entrez Gene: 11335 Human
- Entrez Gene: 12417 Mouse
- Entrez Gene: 297093 Rat
- Omim: 604477 Human
- SwissProt: Q13185 Human
- SwissProt: P23198 Mouse
- SwissProt: Q5RJK5 Rat
- Unigene: 381189 Human
see all
Anti-HP1 gamma (unmodified S93) antibody images:
Western blot - HP1 gamma antibody (ab79304)
All lanes : Anti-HP1 gamma (unmodified S93) antibody (ab79304) at 1/500 dilution
Lane 1 : Extracts from 293 cells with no immunizing peptide
Lane 2 : Extracts from 293 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
References for Anti-HP1 gamma (unmodified S93) antibody (ab79304)
ab79304 has not yet been referenced specifically in any publications.
Publishing research using ab79304? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"