Anti-HP1 gamma (unmodified S93) antibody (ab79304)

Overview

  • Product nameAnti-HP1 gamma (unmodified S93) antibody
    See all HP1 gamma primary antibodies
  • Description
    Rabbit polyclonal to HP1 gamma (unmodified S93)
  • SpecificityDetects endogenous levels of total HP1 gamma protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human HP1 gamma around the phosphorylation site of serine 93 (RKSPLS).

  • Positive control
    • 293 nuclear lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab79304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa.
ELISA 1/40000.

Target

  • FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similaritiesContains 2 chromo domains.
  • Post-translational
    modifications
    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Anti-HP1 gamma (unmodified S93) antibody images

  • All lanes : Anti-HP1 gamma (unmodified S93) antibody (ab79304) at 1/500 dilution

    Lane 1 : Extracts from 293 cells with no immunizing peptide
    Lane 2 : Extracts from 293 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

References for Anti-HP1 gamma (unmodified S93) antibody (ab79304)

ab79304 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Raw 264.7)
Loading amount 40 µg
Specification Raw 264.7
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (CBX3 recombinant protein)
Loading amount 0.1 µg
Specification CBX3 recombinant protein
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

James Thorne

Verified customer

Submitted Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"