Overview

  • Product name
  • Description
    Rabbit polyclonal to HP1BP3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 71 - 120 (SEESVSTVEEQENETPPATSSEAEQPKGEPENEEKEENKSSEETKKDEK D) of Human HP1BP3 (NP_057371).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab98894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity (PubMed:24830416). Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal(PubMed:25100860).
  • Sequence similarities
    Contains 3 H15 (linker histone H1/H5 globular) domains.
  • Domain
    A central region that included the first H15 (linker histone H1/H5 globular) domain binds at the entry/exit site of the nucleosomal DNA.
  • Cellular localization
    Nucleus. Chromosome. localized in nuclei but not in nucleoli in interphase. Colocalized with chromosomes in mitosis, with a gradually increased during G1 progression and a maximum level during late G1 phase (G1/S).
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterochromatin protein 1, binding protein 3 antibody
    • Heterochromatin protein 1-binding protein 3 antibody
    • HP1 BP74 antibody
    • HP1B3_HUMAN antibody
    • HP1BP 3 antibody
    • HP1BP3 antibody
    • MGC28927 antibody
    • MGC43701 antibody
    • MGC72624 antibody
    • OTTHUMP00000002717 antibody
    • OTTHUMP00000002719 antibody
    • OTTHUMP00000002720 antibody
    • Protein HP1 BP74 antibody
    • Protein HP1-BP74 antibody
    • RP5 930J4.3 antibody
    see all

Images

  • Anti-HP1BP3 antibody (ab98894) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 61 kDa

References

This product has been referenced in:
  • Dutta B  et al. Quantitative profiling of chromatome dynamics reveals a novel role for HP1BP3 in hypoxia-induced oncogenesis. Mol Cell Proteomics 13:3236-49 (2014). Read more (PubMed: 25100860) »
  • Dutta B  et al. Profiling of the Chromatin-associated Proteome Identifies HP1BP3 as a Novel Regulator of Cell Cycle Progression. Mol Cell Proteomics 13:2183-97 (2014). Read more (PubMed: 24830416) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - nuclear (HepG2 cells)
Specification
HepG2 cells
Negative control
IgG antibody. CRISPR-Cas9 HP1BP3 KO
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
I used H3 antibody as a positive control for the whole ChIP experiments and as an internal histone occupancy control as I used tandem repeats sequences as a read out.
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Submitted Feb 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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