Overview

  • Product nameAnti-HPD antibody
    See all HPD primary antibodies
  • Description
    Mouse monoclonal to HPD
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: YEAPAFMDPL LPKLPKCSLE MIDHIVGNQP DQEMVSASEW YLKNLQFHRF , corresponding to amino acids 160-270 of Human HPD (NP_002141)

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionKey enzyme in the degradation of tyrosine.
  • PathwayAmino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.
  • Involvement in diseaseTyrosinemia 3
    Hawkinsinuria
  • Sequence similaritiesBelongs to the 4HPPD family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4 HPPD antibody
    • 4 hydroxyphenylpyruvate dioxygenase antibody
    • 4 hydroxyphenylpyruvic acid oxidase antibody
    • 4-hydroxyphenylpyruvate dioxygenase antibody
    • 4-hydroxyphenylpyruvic acid oxidase antibody
    • 4HPPD antibody
    • GLOD3 antibody
    • glyoxalase domain containing 3 antibody
    • HPD antibody
    • HPPD_HUMAN antibody
    • HPPDase antibody
    • PPD antibody
    see all

Anti-HPD antibody images

  • Anti-HPD antibody (ab76569) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • Anti-HPD antibody (ab76569) at 1/500 dilution + partial recombinant protein at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-HPD antibody (ab76569)

ab76569 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"