Recombinant Anti-HPD antibody [EPR5297] (ab133515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5297] to HPD
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HPD antibody [EPR5297]
See all HPD primary antibodies -
Description
Rabbit monoclonal [EPR5297] to HPD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 and Fetal liver lysates. Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.57 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5297 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250-1/50 See IHC antigen retrieval protocols. The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250-1/50 See IHC antigen retrieval protocols. The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples. |
Target
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Function
Key enzyme in the degradation of tyrosine. -
Pathway
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6. -
Involvement in disease
Tyrosinemia 3
Hawkinsinuria -
Sequence similarities
Belongs to the 4HPPD family. - Information by UniProt
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Database links
- Entrez Gene: 3242 Human
- Entrez Gene: 29531 Rat
- Omim: 609695 Human
- SwissProt: P32754 Human
- SwissProt: P32755 Rat
- Unigene: 2899 Human
- Unigene: 3664 Rat
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Alternative names
- 4 HPPD antibody
- 4 hydroxyphenylpyruvate dioxygenase antibody
- 4 hydroxyphenylpyruvic acid oxidase antibody
see all
Images
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Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1:2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution (purified) + Rat liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133515 has been referenced in 1 publication.
- Johnston K et al. Isotope tracing reveals glycolysis and oxidative metabolism in childhood tumors of multiple histologies. Med 2:395-410 (2021). PubMed: 33860280