Application notesWB: 1/250. Detects a band of approximately >250 kDa (predicted molecular weight: 121 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSubunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) [MIM:608233]. Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS. HPS2 differs from the other forms of HPS in that it includes immunodeficiency in its phenotype and patients with HPS2 have an increased susceptibility to infections.
Sequence similaritiesBelongs to the adaptor complexes large subunit family.
Post-translational modificationsPhosphorylated on serine residues.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Golgi apparatus. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Adapter related protein complex 3 beta 1 subunit antibody
Adapter-related protein complex 3 subunit beta-1 antibody
Adaptor protein complex AP-3 subunit beta-1 antibody
Adaptor protein complex AP3 beta1 subunit antibody
AP-3 complex subunit beta-1 antibody
AP3 complex beta1 subunit antibody
Beta3A adaptin antibody
Clathrin assembly protein complex 3 beta 1 large chain antibody
Clathrin assembly protein complex 3 beta-1 large chain antibody
Anti-HPS2 antibody images
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Western blot - Anti-HPS2 antibody (ab71169)
All lanes : Anti-HPS2 antibody (ab71169) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Rat Thymus Tissue Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Liver (Mouse) Tissue Lysate Lane 5 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution