Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)

Overview

  • Product nameAnti-HPV antibody [BPV-1/1H8 + CAMVIR]
    See all HPV primary antibodies
  • Description
    Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV
  • SpecificityThe antibody detects the HPV-1, 6,11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. This antibody has been tested in ELISA with purified BPV-1 major capsid protein.
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Immunogen

    HPV Cocktail Broad Spectrum (HPV-1, 6, 11, 16-16, 18 and 31)

  • Positive control
    • Infected cervical biopsy.
  • General notesThis antibody is broad-spectrum cocktail of two clones - BPV-1/1H8 (IgG) and CAMVIR (IgG2a)


    The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.30
    Preservatives: Proprietary preservative, 0.09% Sodium azide
    Constituents: PBS, Tissue culture supernatant
  • Concentration information loading...
  • Primary antibody notesThe broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
  • ClonalityMonoclonal
  • Clone numberBPV-1/1H8 + CAMVIR
  • Myelomaunknown
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent concentration. PubMed: 22479413

Target

  • RelevanceHuman papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer.
  • Cellular localizationNuclear
  • Alternative names
    • HPV antibody
    • Human papillomavirus antibody

Anti-HPV antibody [BPV-1/1H8 + CAMVIR] images

  • ab2417 staining HPV-infected human cervix tissue sections.

References for Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)

This product has been referenced in:
  • Roperto S  et al. Productive infection of bovine papillomavirus type 2 in the placenta of pregnant cows affected with urinary bladder tumors. PLoS One 7:e33569 (2012). WB . Read more (PubMed: 22479413) »
  • Roperto S  et al. PBMCs are additional sites of productive infection of bovine papillomavirus type 2. J Gen Virol 92:1787-94 (2011). ICC/IF . Read more (PubMed: 21525209) »

See all 2 Publications for this product

Product Wall

I recommend ab97023, goat anti-mouse IgG H&L (heavy and light chains), assuming you want an HRP conjugate (horseradish peroxidase) for visualization. We also have fluorochrome conjugates and alkaline phosphatase conjugates, among others.

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Tobacco Recombinant protein (Plant cell)
Loading amount 21 µg
Specification Plant cell
Gel Running Conditions Reduced Denaturing (gel 12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Mr. Diego Grando Módolo

Verified customer

Submitted Dec 18 2012

Thank you for contacting Abcam

Theprotein carrier forHPV Cocktail Broad Spectrumis heat attenuated goat serum.

Please let me know if there is anything else I can help you with.

Thank you for your enquiry and for your patience. It has taken some time to gather the information.

Regardnig ab2417 Anti-HPV antibody [BPV-1/1H8 + CAMVIR], I can confirm thiswas raised against the major capsid protein L1. I have copied belo...

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Thank you for contacting us.

My colleague has created an SDS for ab2417 and it is now available for download from our website. I have also attached it to this email. Please let me know if you need any additional information.

Thanks you for your enquiry. Based on the following publications, there are cases where the HPV expression was found in the cytoplasm and nuclear region. 1) CASE REPORT: Corneal papilloma associated with papillomavirus in a one-humped camel (Ca...

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Thank you for your enquiry regarding ab2417. After discussing your comments with some experts, we have reviewed the alternative names on the datasheet and amended it. We apologize for any inconvenience caused and thank you for bringing this matte...

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Thank you for your enquiry. This antibody was used at a 1:80 dilution and the secondary antibody was a MACH3 Mouse AP Polymer. Please bear in mind that different samples and buffers used may require some optimization. I hope this information wi...

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The antibody was used at a concentration of 1:80 in IHC-P. Secondary was AP-polymer. We have not carried out ICC/IF for this antibody. Thus, we are unable to guarantee the suitability of this application. Probably antibody could be further diluted.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"