Overview

  • Product nameAnti-HPV11 E2 antibody [10E12]
  • Description
    Mouse monoclonal [10E12] to HPV11 E2
  • SpecificityReacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human Papillomavirus (HPV)
  • Immunogen

    Recombinant full length HPV11 E2 protein purified from bacteria.

  • EpitopeMapped to amino acids 202-284
  • Positive control
    • U20S or Hela cells and lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab100968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 42 kDa.
ELISA 1/32000.
ICC/IF Use at an assay dependent dilution.
  • Application notesIs unsuitable for IP.
  • Target

    • RelevanceHuman papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
    • Cellular localizationHost nucleus
    • Database links
      • Alternative names
        • Human papillomaviruses 11 E2 antibody

      Anti-HPV11 E2 antibody [10E12] images

      • All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution

        Lane 1 : lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 2 : lysate of plain U20S cells
        Lane 3 : lysate of plain HeLa cells
        Lane 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 5 : marker


        Predicted band size : 42 kDa
      • Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
        IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.

      References for Anti-HPV11 E2 antibody [10E12] (ab100968)

      ab100968 has not yet been referenced specifically in any publications.

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