Anti-HPV16 E1+E4 antibody [TVG 402] (ab20189)

Overview

  • Product nameAnti-HPV16 E1+E4 antibody [TVG 402]
    See all HPV16 E1+E4 primary antibodies
  • Description
    Mouse monoclonal [TVG 402] to HPV16 E1+E4
  • SpecificityRecognises the human papilloma virus type 16 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, IHC-Frmore details
  • Species reactivity
    Human papilloma virus.
  • Immunogen

    Fusion protein:

    PWAPKK

    , corresponding to amino acids 36-41 of HPV16 E1+E4. Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.

  • EpitopeAmino acids 36-41.
  • General notes


    This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Properties

Applications

Our Abpromise guarantee covers the use of ab20189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceHPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.
  • Cellular localizationNuclear
  • Alternative names
    • ATP dependent helicase E1 antibody
    • E1 antibody
    • E4 antibody
    • HPV16 E1 antibody
    • HPV16 E4 antibody
    • Human papillomavirus type 16 E1 antibody
    • Human papillomavirus type 16 E4 antibody
    • Replication protein antibody
    • Replication protein E1 antibody
    see all

References for Anti-HPV16 E1+E4 antibody [TVG 402] (ab20189)

ab20189 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Cow Cultured Cells (Epithelial cells of cow with BPV)
Specification Epithelial cells of cow with BPV
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 (0,1%), Time: 10min
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2012

Thank you for the information and the reference link. I confess ignorance about the clone; I assumed our notation "E1+E4" meant reactivity with those two early antigens, not just E4. I do see that we list IHC as a tested application. My concern was tha...

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Thank you for contacting us.

My first thought was that nuclear staining should be expected, in addition to cytoplasmic, but I do not know if there is a consensus on this. I found the following article which discusses in situ hybridization da...

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I am very pleased to hear you would like to accept our offer and test ab20189 in ICC in bovine sample. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this...

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DISCOUNT CODE:100%ABR-T713U

Expiration date: 22/04/2012

I am very pleased to hear you would like to useab20189 inIHC-P and send us an image of your results.

This code will give you 1 free PRIMARY ANTIBODY before the expi...

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Thank you very much for your interest in ab20189.



I can confirm that this antibody (Ref:ab20189: Anti-HPV16 E1+E4 antibody [TVG 402]) has been tested and characterized for the use in immunohistochemistry both on frozen and on forma...

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Thank you for your enquiry and your interest in our products.

I can confirm that this antibody (Ref:ab20189: Anti-HPV16 E1+E4 antibody [TVG 402]) has been tested and characterized for the use in immunohistochemistry both on frozen and on form...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"