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PWAPKK, corresponding to amino acids 36-41 of HPV16 E1+E4. Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.
This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.
Our Abpromise guarantee covers the use of ab20189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab20189 has not yet been referenced specifically in any publications.