Overview

  • Product name
    Anti-HPV16 E2 antibody [TVG 261]
    See all HPV16 E2 primary antibodies
  • Description
    Mouse monoclonal [TVG 261] to HPV16 E2
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with HPV E2.
  • Immunogen

    Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2 (generated from an expression vector in which the E2 ORF was cloned in frame with malE).

  • Epitope
    aa2-17 - ETLCQRLNVCQDKILT.
  • General notes


    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.
  • Clonality
    Monoclonal
  • Clone number
    TVG 261
  • Myeloma
    NS0/1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

Target

  • Alternative names
    • HPV16 regulatory protein E2 antibody
    • Human papilloma virus 16 E2 antibody
    • Human papilloma virus 16 regulatory protein E2 antibody
    • Human papillomavirus type 16 E2 antibody
    • Human papillomavirus type 16 regulatory protein E2 antibody
    • Regulatory protein E2 antibody
    see all

References for Anti-HPV16 E2 antibody [TVG 261] (ab17185)

This product has been referenced in:
  • Harris L  et al. The Cellular DNA Helicase ChlR1 Regulates Chromatin and Nuclear Matrix Attachment of the Human Papillomavirus 16 E2 Protein and High-Copy-Number Viral Genome Establishment. J Virol 91:N/A (2017). WB . Read more (PubMed: 27795438) »
  • Sharma R & Palefsky JM Addition of a single E2 binding site to the human papillomavirus (HPV) type 16 long control region enhances killing of HPV positive cells via HPV E2 protein-regulated herpes simplex virus type 1 thymidine kinase-mediated suicide gene therapy. Hum Gene Ther 21:843-54 (2010). WB . Read more (PubMed: 20132050) »

See all 4 Publications for this product

Product Wall

Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me ...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
300000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2010

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos)
Loading amount
500000 cells
Specification
Cos
Treatment
Transfected with MV11.HPV16E2
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Clare Davy

Verified customer

Submitted Sep 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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