Overview

  • Product nameAnti-HPV16 E2 antibody [TVG 261]
    See all HPV16 E2 primary antibodies
  • Description
    Mouse monoclonal [TVG 261] to HPV16 E2
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with HPV E2.
  • Immunogen

    Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2 (generated from an expression vector in which the E2 ORF was cloned in frame with malE).

  • Epitopeaa2-17 - ETLCQRLNVCQDKILT.
  • General notes


    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesThe human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.
  • ClonalityMonoclonal
  • Clone numberTVG 261
  • MyelomaNS0/1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

Target

  • Alternative names
    • HPV16 regulatory protein E2 antibody
    • Human papilloma virus 16 E2 antibody
    • Human papilloma virus 16 regulatory protein E2 antibody
    • Human papillomavirus type 16 E2 antibody
    • Human papillomavirus type 16 regulatory protein E2 antibody
    • Regulatory protein E2 antibody
    see all

References for Anti-HPV16 E2 antibody [TVG 261] (ab17185)

This product has been referenced in:
  • Sharma R & Palefsky JM Addition of a single E2 binding site to the human papillomavirus (HPV) type 16 long control region enhances killing of HPV positive cells via HPV E2 protein-regulated herpes simplex virus type 1 thymidine kinase-mediated suicide gene therapy. Hum Gene Ther 21:843-54 (2010). WB . Read more (PubMed: 20132050) »
  • Fernandez AF  et al. The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer. Genome Res 19:438-51 (2009). WB . Read more (PubMed: 19208682) »

See all 3 Publications for this product

Product Wall

Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 300000 cells
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2010

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos)
Loading amount 500000 cells
Specification Cos
Treatment Transfected with MV11.HPV16E2
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. clare davy

Verified customer

Submitted Sep 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"