Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)


  • Product name
    Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]
    See all HPV16 E6 + HPV18 E6 primary antibodies
  • Description
    Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Immunogen

    Gel-purified HPV-18 E6-beta galactosidase fusion protein.

  • Positive control
    • Human cervical cancer cells.
  • General notes

    Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

    The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.



Our Abpromise guarantee covers the use of ab70 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.

16.5 kDa band is HPV-18, 17 kDa band is HPV-16.

The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • Relevance
    Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
  • Alternative names
    • E6 antibody
    • Human Papilloma Virus antibody
    • Human papillomavirus type 16 E6 antibody
    • Human papillomavirus type 18 E6 antibody
    • Protein E6 antibody
    see all


  • Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200


This product has been referenced in:
  • Wang X  et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28480962) »
  • Xi R  et al. HPV16 E6-E7 induces cancer stem-like cells phenotypes in esophageal squamous cell carcinoma through the activation of PI3K/Akt signaling pathway in vitro and in vivo. Oncotarget 7:57050-57065 (2016). WB . Read more (PubMed: 27489353) »

See all 14 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry

I heard back from the lab that, unfortunately, they do not have any information relating to the structure of the antibody. But this is a standard antibody so I would expect that is does have an Fc region.


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Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

Here is the discount...

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Thank you for contacting us and your interest in our products.

The antibodies ab70 and ab30716 are in fact the same antibody, produced from different sources.I can understand that this is currently not reflected very well on our website but w...

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Abcam has not validated the combination of species/application used in this Abreview.
Cow Cultured Cells (Epithelial cells of cows with BPV and cells free-B)
Epithelial cells of cows with BPV and cells free-B
Yes - Tritonx100 (0,1%),
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 31 2012

Thank you for your reply.

I have located the Abreview in our system for ab70. It seems that the customer has begun this review, but must provide the product lot number (found on the vial) in order to officially submit the review. Therefor...

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Thank you for the update and I am really happy that the customer was satisfied with the outcome.

If you have any other situations like this or if there is anything else we can help with, then please let me know.

Thank you very much for contacting us a while back about the HPV16E6 + HPV18 E6 antibody ab70.

I sincerely apologize for the extended delay in getting this information for you, and I appreciate your patience while I have been in touch with th...

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Thank you for your reply.

As we have not previously tested this antibody in ICC/IF, we could not guarantee that it would work for this application, as under our Abpromise we only guarantee it to work in WB, IP& IHC-P.

However, I ...

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Thank you very much for your interest in (Anti-HPV16 E6 + HPV18 E6 antibody [C1P5])

To our knowledge,ab70 has not been tested with p16ink4a. Therefore, I can offer a discount off a future purchase if you buy ab70 now,...

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I am afraid we do not have the immunogen sequence information for this antibody. I would suggest the following publications as some extra information for your customer but otherwise I cannot help on this occasion.

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